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KMID : 1161420130160050454
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Journal of Medicinal Food
2013 Volume.16 No. 5 p.454 ~ p.459
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Biochemical Composition, Antimicrobial Activities,and Anti?Quorum-Sensing Activities of Ethanol and Ethyl Acetate Extracts from Hypericum connatum Lam. (Guttiferae)
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Fratianni Florinda
Nazzaro Filomena
Marandino Aurelio
del Rosario Fusco Maria
Coppola Raffaele
De Feo Vincenzo
De Martino Laura
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Abstract
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We studied the polyphenol composition and antioxidant activity of the ethanolic and ethyl acetate extracts of Hypericum connatum (Guttiferae), in addition to their antimicrobial and antiquorum-sensing (AQS) properties. The extracts exhibited a similar polyphenol content and a notable antioxidant activity, which was stronger in the ethanolic extract (EC50=3.2 μg/mL). Ultrahigh-performance liquid chromatography analyses revealed different polyphenol profiles, with rutin and apigen being the main components of the ethanolic extract and caffeic acid, (?)-epicatechin and p-coumaric acid being abundant in the ethyl acetate extract. The ethyl acetate extract exhibited a more effective antimicrobial activity than the ethanolic extract against the pathogens Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa. For the first time, AQS activity was detected in H. connatum: the ethanolic extract inhibited the production of violacein, a quorum-sensing?regulated pigment, in a Chromobacterium violaceum tester strain, without interfering with its growth.
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KEYWORD
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antimicrobial activity, DPPH assay, Hypericum connatum, polyphenols, quorum sensing
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